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Image Search Results
Journal: International Journal of Nanomedicine
Article Title: A peptide-mediated targeting gene delivery system for malignant glioma cells
doi: 10.2147/IJN.S44990
Figure Lengend Snippet: Internalization of nanoparticles. Notes: U-87 cell was incubated with the PV 20 nanoparticles containing the FITC-labeled pDNA. Lysosome was marked with Lyso-Tracker Red (Beyotime, Haimen, People’s Republic of China) to investigate the location of nanoparticles in cytoplasm. The nucleus was stained with Hoechst 33342 (Beyotime, Haimen, People’s Republic of China). Internalization assays showed that PV 20 nanoparticles penetrated into U-87 cells, were mostly co-localized with lysosomes at 1 hour after the beginning of transfection, and escaped successfully from lysosomes at 4 hours. Competition assay showed there was very little internalization of PV 20 nanoparticles in U-87 cells due to the block effect by free VTW peptide. Scale bar: 50 μm. Abbreviations: FITC, fluorescein isothiocyanate; pDNA, plasmid DNA; PV, VTW-modified PEI copolymers; VTW, VTWTPQAWFQWV.
Article Snippet: The
Techniques: Incubation, Labeling, Staining, Transfection, Competitive Binding Assay, Blocking Assay, Plasmid Preparation, Modification